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Citations Search

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J. Immunol. 160, 4353-4360. Regulation of transcription of the TATA-less human complement component C4 gene. 1998

Vaishnaw, A. K. , Mitchell, T. J. , Rose, S. J. , Walport, M. J. , Morley, B. J.

Notes: The authors used the Primer Extension System - AMV reverse transcriptase to determine transcription efficiencies of transfected constructs. Also, the fmol® DNA Cycle Sequencing System was used to confirm the nature of the constructs. EMSAs were carried out using Promega's SP1 Oligonucleotides. (0212)

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J. Biol. Chem. 273, 14885-14890. Transcriptional regulation of endothelial nitric-oxide synthase by lysophosphatidylcholine 1998

Cieslik, K., Zembowicz, A., Tang, J.-L., Wu, K.K.

Notes: The Serine/Threonine Phosphatase Assay System was used to assess protein phosphatase 2A, 2B and 2C activity in nuclear extracts of Human umbilical vein cells (HUVEC) with or without lysophosphatidylcholine treatment. Gel shifts were also performed with nuclear extracts prepared from the HUVEC cells using the SP1 Consensus Oligonucleotide. Luciferase reporter studies were also performed in HUVEC cells using pGL3 Basic-derived vectors and the Luciferase Assay System. (1305)

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J. Biol. Chem. 272, 14244-14250. Transcription factor AP-2 controls transcription of the human transforming growth factor-alpha gene. 1997

Wang, D., Shin, T. H., Kudlow, J. E.

Notes: Luciferase studies were performed with MDA468 human mammary carcinoma cells using constructs prepared in the pGL2-Basic Vector. Gel shift assays were performed with extracts of these cells using the AP2 and SP1 Consensus Oligonucleotides. (0197)

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J. Biol. Chem. 272, 6051-6058. Tyrosine hydroxylase gene promoter activity is regulated by both cyclic AMP-responsive element and AP1 sites following calcium influx. Evidence for cyclic amp-responsive element binding protein-independent regulation. 1997

Nagamoto-Combs, K., Piech, K.M., Best, J.A., Sun, B., Tank, A.W.

Notes: The pCAT® Basic Vector was used to measure baseline CAT activity in PC12 cells. The Sp1 Consensus Oligonucleotide was used for gel shifts from transfected PC12 cells. (The pCAT® Basic Vector has been replaced by the next generation vector, pCAT®3 Basic.) (0640)

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Anal. Biochem. 213, 162-167. Enhanced gel mobility shift assay for DNA-binding factors. 1993

Hassanain, H.H., Dai, W., Gupta, S.L.

Notes: The authors used AP1 and SP1 Consensus Oligonucleotides in an improved binding buffer containing detergents. (1068)

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