Broudy, V.C., Lin, N.L., Sabath, D.F., Papayannopoulou, T. and Kaushansky, K.
Notes: In this study, Poly(A)+ RNA was isolated from B16 melanoma cell total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for cDNA library construction. The RNAgents® Total RNA Isolation System was used to isolate total RNA from HEL and K562 human erythroleukemia cells. The isolated RNA was used for RT-PCR. RNasin® Ribonuclease Inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. The lysates from these cells were used directly for RT-PCR. (2026)