Bergmann, M., Hart, L., Lindsay, M., Barnes, P.J. and Newton, R.
Notes: IL-1β and TNFα have been shown to induce two p50/p65 NF-κB DNA binding complexes in A549 cells. Tfx™-50 Reagent was used to stably transfect A549 cells in order to study the effects on NF-κB-dependent transcription. Promega's Luciferase Assay System was used to measure the effect of kinase inhibitors on IL-1β and TNFα stimulated NF-κB-dependent transcription. In addition, Promega's NF-κB and OCT1 Oligonucleotides were used in gel shift assays (1438)