United States

United States

Choose language: English

Citations Search

Sort By:

Forensic Sci. Int. Genet. 2, 301–309. Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer. 2008

Liu, P. Yeung, S.H.I., Crenshaw, K.A., Crouse, C.A., Scherer, J.R. and Mathies, R.A.

Notes: This paper describes analysis of DNA samples at a mock crime scene using automated DNA purification followed by STR analysis on a portable microchip system. The mock crime scene was created using "victim" and "suspect" blood stains prepared by spotting 3µl of liquid blood onto paper towels and a cloth shirt. The samples were allowed to dry overnight before placement at the crime scene. Samples were processed at the scene using the Maxwell® 16 Instrument and DNA IQ™ Casework Sample Kit for DNA extraction, and a microchip analyzer to perform amplification and STR analysis. The 9-plex autosomal STR typing system used in the microchip system included primer sequences from the PowerPlex® 16 System (D3S1358, THO1, D21S11, D5S818, D13S317, D7S820, vWA and D8S1179) and amelogenin for sex identification. DNA purification from suspect samples was completed in 2 hours, and subsequent STR analysis and generation of a "suspect" DNA profile took a further 3 hours. The entire process from sample collection to generation of a CODIS "hit" took 6 hours. (3922)

Expand Full Notes »

J. Cell Sci. 116, 2421-2430. PTHrP [67–86] regulates the expression of stress proteins in breast cancer cells inducing modifications in urokinase-plasminogen activator and MMP-1 expression. 2003

Luparello, C., Sirchia, R. and Pupello, D.

Notes: Total and poly(A)+ RNA from treated and untreated 8701-BC breast cancer tumor cells were treated with RQ1 RNase-free DNase. The RNA samples were reverse transcribed using the M-MLV RNase H– point mutant reverse transcriptase and random primers to create cDNA used in downstream analyses. The cDNA from the reactions were used in PCR, differential display PCR, and semi-quantitative multiplex PCR reactions. In differential display PCR studies, the authors analyzed differentially displayed bands by staining 6% polyacrylamide gels with the SILVER SEQUENCE™ Staining Reagents. Differentially displayed bands were re-amplified and cloned using the pGEM®-T Easy Vector and high efficiency JM109 competent cells. (3020)

Expand Full Notes »

Genetics 153, 1743-1751. Insights into genome differentiation: pheromone-binding protein variation and population history in the European corn borer (Ostrinia nubilalis). 1999

Willett, C. S., Harrison, R. G.

Notes: In this paper, DNA visualized on a sequencing gel is stained with the SILVER SEQUENCE™ DNA Sequencing Systems, and PCR products are cloned into the pGEM®-T Vector. (0187)

Expand Full Notes »

Genetics 151, 785-795. Multiple-trait quantitative trait loci analysis using a large mouse sibship. 1999

Jackson, A.U., Fornes, A., Galecki, A., Miller, R.A., Burke, D.T.

Notes: 20ng mouse genomic DNA was amplified with primers specific for 83 SSLP (single sequence length polymorphism) marker loci for genome-wide genotyping. Amplification products were separated on a denaturing gel and were visualized by silver staining using the SILVER SEQUENCE™ DNA Sequencing System. (0965)

Expand Full Notes »

Cell 92, 63-72. Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. 1998

Gleeson, J.G., Allen, K.M., Fox, J.W., Lamperti, E.D., Berkovic, S., Scheffer, I., Cooper, E.C., Bobyns, W.B., Minnerath, S.R., Ross, M.E., Walsh, C.A.

Notes: The SILVER SEQUENCE™ Staining Reagents were used to stain single-strand conformation polymorphism (SSCP) reactions separated on a 6% polyacrylamide gel. Aberrant bands were excised and the DNA was eluted. The isolated DNA was reamplified and characterized by sequence analysis. (1106)

Expand Full Notes »

Choose your country


United States

Pacific Asia

Korea, Republic of


United Kingdom