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Nucl. Acids Res. 31, 981-987. siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells. 2003

Kawasaki, H., Suyama, E., Iyo, M., and Taira, K.

Notes: The PinPoint™ Xa Protein Purification System was used to clone and produce recombinant human Dicer (re-hDicer). Blunt-ended cDNA coding hDicer was cloned into one of the PinPoint™ Xa vectors. Biotinylated re-hDicer was produced in E. coli by inducing cultures with 100μM IPTG in the presence of 2μM biotin. Recombinant hDicer was purified from E. coli lysates with SoftLink™ Soft Release Avidin Resin. The purified re-hDicer was demonstrated to have a putative molecular weight of ~220KDa. Recombinant-hDicer protein was also demonstrated to have RNase III-like activity by a processing assay using double-stranded puromycin resistance gene mRNA. (2706)

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EMBO J. 22(5), 1067-1074. The human chromokinesin Kid is a plus end-directed microtubule-based motor. 2003

Yajima, J., Edamatsu, M., Watai-Nishii, J., Tokai-Nishizumi, N., Yamamoto, T. and Toyoshima, Y.Y.

Notes: The the motor function of the DNA-binding kinesin-like protein Kid was studied. Biotinylated Kid domains were expressed using the PinPoint™ System and the motor force of  Kid pulling on an optically trapped streptavidin bead was detected.  (2703)

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J. Virol. Methods 92, 121–9. A simple procedure for expression and purification of selected non-structural (alpha and beta) herpes simplex virus 1 (HSV-1) proteins. 2001

Kosovský, J., Durmanová, V., Kúdelová, M., Rezuchová, I., Tkáciková, L. and Rajcáni, J.

Notes: The authors used the PinPoint™ Xa-1 Vector to clone two herpes simplex virus (HSV) genes and express the encoded proteins, IE63 and thymidine kinase, in E. coli JM109 for use as immunogens. Proteins expressed from the PinPint™ Vectors have a biotinylated tag, and the authors used this tag to purify the HSV proteins using the SoftLink™ Soft Release Avidin Resin. In a large-scale purification, the typical yield of IE63 protein was ~650µg/L. The recommended PinPoint™ purification protocol was modified in several ways: 1) the optimal incubation temperature and time for protein induction in E. coli cultures was chosen based on JM109 growth curves; 2) the post-induction time was optimized by monitoring protein expression levels using Western blot analysis; 3) the length of time that proteins were allowed to bind to the SoftLink™ Resin was optimized. The authors found that shortening the length of binding time to 2 hours eliminated the co-purification of endogenous biotinylated proteins in E. coli. (3850)

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Eur. J. Biochem. 261, 137-147. Identification of chURP, a nuclear calmodulin-binding protein related to hnRNP-U. 1999

Lodge, A.P., Walsh, A., McNamee, C.J., Moss, D.J.

Notes: The chURP protein was expressed from the PinPoint™ Xa-3 Vector in a 2-liter culture of JM109. Much detail is provided for purification of the protein to make an immunogen for antibody production. (0741)

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J. Clin. Microbiol. 36, 2514-2521. Antigenic characterization of Hantaan and Seoul virus nucleocapsid proteins expressed by recombinant baculovirus: Application of a truncated protein, lacking an antigenic region common to the two viruses as a serotyping antigen. 1998

Morii, M., Yoshimatsu, K., Arikawa, J., Zhou, G., Kariwa, H., Takashima, I.

Notes: The PinPoint® Xa System was used to produce recombinant Hantaan virus nucleocapsid proteins. The methods used in the production of the recombinant proteins is found in the following journal article: Yoshimatsu, K, et al. (1996) J. Gen. Virol. 77, 695-704. (0662)

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Science 282, 2088-2092. Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors. 1998

Nguyen, J.T. , Turck, C.W. , Cohen, F.E. , Zuckermann, R.N. , Lim, W.A.

Notes: The authors used the PinPoint™ Xa Protein Purification System to express the Grb2 SH3 domain for competitive inhibition studies of peptides that interfere with its interaction with the proline-rich domain of sos. (0607)

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Proc. Natl. Acad. Sci. USA 95, 8087-8092. Identification of a human PTS1 receptor docking protein directly required for peroxisomal protein import. 1998

Fransen, M., Terlecky, S.R., Subramani, S.

Notes: The human protein hsPex14p as well as another protein hsPex13p and hsPex5p were cloned into the PinPoint® Xa2 vector and expressed in JM109 E. coli. The cell extracts were run on SDS PAGE gels, transferred to nitrocellulose and probed with the anti-MF3 antisera. The hsPex5p, supposedly the human PTS1 homolog, was translated in vitro with the TNT® Coupled Reticulocyte Lysate System and reacted with another blot with the same proteins and the hsPex14p bound the 35S-labeled protein. (1172)

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Neuron 21, 727-739. SAP90 binds and clusters kainate receptors causing incomplete desensitization. 1998

Garcia, E. P. , Mehta, S. , Blair, L. A. , Wells, D. G. , Shang, J. , Fukushima, T. , Fallon, J. R. , Garner, C. C. , Marshall, J.

Notes: Used PinPoint™ Xa-3 vector to express C-terminal segments of GluR6. (1139)

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J. Biol. Chem. 273, 33635-33643. The difference in recognition of terminal tripeptides as peroxisomal targeting signal 1 between yeast and human is due to different affinities of their receptor Pex5p to the cognate signal and to residues adjacent to it. 1998

Lametschwandtner, G., Brocard, C., Fransen, M., Van Veldhoven, P., Berger, J., Hartig, A.

Notes: The authors used PinPoint™ Xa-1 Vector to express a biotinylated Pex5p protein for an in vitro ELISA-like assay. (0833)

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Blood 89, 2105-2112. 1,25-dihydroxyvitamin D3 induces NAD(+)-dependent 15- hydroxyprostaglandin dehydrogenase in human neonatal monocytes. 1997

Pichaud, F., Roux, S., Frendo, J. L., Delage-Mourroux, R., Maclouf, J., de Vernejoul, M.C., Moukhtar, M.S., Jullienne, A.

Notes: The PinPoint™ Protein Purification System was used to express a 178 kDa protein. The protein was expressed, purified on the SoftLink™ Avidin and the purification tag cleaved with Factor Xa. (0563)

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Cell 90, 1107-1112. RNA helicase A mediates association of CBP with RNA polymerase II 1997

Nakajima, T., Uchida, C., Anderson, S.F., Lee, C.-G., Hurwitz, J., Parvin, J.D., Montiminy, M.

Notes: Eighty-five residue of CBP were inserted into the PinPoint™ Xa-1 Vector, expressed and purified on a monomeric avidin column. The purified protein was used to screen a human placenta cDNA expression library with the aid of streptavidin-alkaline phosphatase. Two clones were identified out of 3 million plaques. Both clones encoded the RNA helicase A. (0643)

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Neuron 17, 255-265. SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo. 1996

Muller, B.M., Kistner, U., Kindler, S., Chung, W.J., Kuhlendahl, S., Fenster, S.D., Lau, L.-F., Veh, R.W., Huganir, R.L., Gundelfinger, E.D., Garner, C.C.

Notes: The nine carboxyl terminal amino acids of NR2B, a subunit of the NMDA receptor, were fused into the PinPoint™ Xa3 Vector. The protein was expressed in DH5α and purified with the SoftLink™ Soft Release Avidin Resin. The purified protein was used to probe various bacterially-expressed fusion protein immobilized on nitrocellulose. Proteins containing the PDZ3 domain interacted with the biotinylated fusion protein and were visualized with a streptavidin-HRP conjugate. (0670)

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Proc. Natl. Acad. Sci. USA 93, 14934-14939. ZnT-3, a putative transporter of zinc into synaptic vesicles 1996

Palmiter, R.D., Cole, T.B., Quaife, C.J., Findley, S.D.

Notes: The c-terminal 93 amino acids of ZnT-3 were expressed from a PinPoint™ Vector and the fusion protein was immobilized on an avidin column. The column-bound protein was used for affinity purification of rabbit immunoglobulins raised against the same peptide fused to maltose binding protein. (0580)

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Infect. Immun. 62, 3424-3433. Cloning and sequence analysis of a chymotrypsinlike protease from Treponema denticola. 1994

Arakawa, S. and Kuramitsu, H.K.

Notes: The 273 amino acid prtB cDNA was cloned into the PinPoint™ Xa-3 Vector and expressed in E. coli HB101 cells. The protein was purified using the SoftLink™ Soft Release Avidin Resin. The purified protein was treated with Factor Xa Protease. Both the fusion protein and the Factor Xa cleaved protease were functional enzymes. (1481)

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