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Promega Corporation

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J. Biol. Chem. 272, 2969-2976. Selective reporter expression in mast cells using a chymase promoter. 1997

Liao, Y., Yi, T., Hoit, B.D., Walsh, R.A., Karnik, S.S., Husain, A.

Notes: The β-Galactosidase gene was excised from the pSV-β-Galactosidase Control Vector and used to make a construct for the production of a transgenic mouse line. The expressed β-Galactosidase protein was detected in mouse tissues by a enzymatic histochemical method. The mRNA of the enzyme was also measured in various tissues and normalized to GAPDH. Promoter activity was studied in COS cells. (0804)

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J. Mol. Neurosci. 8, 63-73. Sequences in the proximal 5´ flanking region of the rat neuron-specific enolase (NSE) gene are sufficient for cell type-specific reporter gene expression. 1997

Twyman, R.M. and Jones, E.A.

Notes: In this study, regulation of the rat neuron-specific enolase gene was examined. The pCAT®-Basic and pCAT®-Control Vectors, and the pSV-β-Galactosidase Control Vector were used. Studies were performed in LTK-, Neuro2A, HeLa, and PC12 cells. The pCAT® Vectors have now been replaced with the improved pCAT®-3 Vector series. (1546)

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J. Biol. Chem. 272, 18418-18424. Syndecan-1 expression is down-regulated during myoblast terminal differentiation. Modulation by growth factors and retinoic acid. 1997

Larrain, J., Cizmeci-Smith, G., Troncoso, V., Stahl, R.C., Carey, D.J., Brandan, E.

Notes: Reporter studies were performed in the mouse skeletal muscle cell line C2C12 using constructs prepared in the pCAT®-Basic Vector. All DNA for transfection was purified by Wizard® Plus Maxipreps DNA Purification System. The pCAT®-Basic Vector has been discontinued. pCAT®3-Basic Vector is available. (0845)

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J. Biol. Chem. 272, 21575-21581. The mouse extracellular signal-regulated kinase 2 gene. Gene structure and characterization of the promoter 1997

Sugiura, N., Suga, T., Ozeki, Y., Mamiya, G., Takishima, K.

Notes: CAT studies were performed in CHO-K1 cells using constructs prepared in the pCAT® Basic Vector. Promega's pCAT® Vectors have now been replaced by the improved pCAT®-3 Vector series. (0314)

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J. Biol. Chem. 272, 5800-5804. The pancreatitis-associated protein I promoter allows targeting to the pancreas of a foreign gene, whose expression is up-regulated during pancreatic inflammation. 1997

Dusetti, N.J. , Vasseur, S. , Ortiz, E.M. , Romeo, H. , Dagorn, J.C. , Burrone, O. , Iovanna, J.L.

Notes: The pCAT® Basic Vector was used to construct recombinant Adenovirus reporter vectors. The recombinant virii were used to study promoter activity in the pancreatic cell line AR-42J. Cells were lysed with Reporter Lysis Buffer for assay. (1208)

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J. Biol. Chem. 272(6), 3430-3436. Transcriptional regulation of the human PAX6 gene promoter. 1997

Xu, Z.P. and Saunders, G.F.

Notes: PolyA+ RNA was isolated from U87, K562, 293, HepB3, HeLa, NIH3T3 and lymphoblastiod total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for Northern analysis. Expression of CAT reporter constructs, derived from the pCAT® Basic Vector, was studied in the U87, K562 and HeLa cells and normalized to control beta-galactosidase. (1698)

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J. Biol. Chem. 272, 6051-6058. Tyrosine hydroxylase gene promoter activity is regulated by both cyclic AMP-responsive element and AP1 sites following calcium influx. Evidence for cyclic amp-responsive element binding protein-independent regulation. 1997

Nagamoto-Combs, K., Piech, K.M., Best, J.A., Sun, B., Tank, A.W.

Notes: The pCAT® Basic Vector was used to measure baseline CAT activity in PC12 cells. The Sp1 Consensus Oligonucleotide was used for gel shifts from transfected PC12 cells. (The pCAT® Basic Vector has been replaced by the next generation vector, pCAT®3 Basic.) (0640)

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Proc. Natl. Acad. Sci. USA 93, 14569-14573. The Gfi-1 protooncoprotein represses Bax expression and inhibits T-cell death. 1996

Grimes, H. L. , Gilks, C. B. , Chan, T. O. , Porter, S. , Tsichlis, P. N.

Notes: Uses Promega pCAT® Basic Vector in their studies. (1121)

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Proc. Natl. Acad. Sci. USA 93, 12845-12850. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3. 1996

Yang, W. M. , Inouye, C. , Zeng, Y. , Bearss, D. , Seto, E.

Notes: The pGEM®-7zf(-) vector was used for subcloning cDNAs. The pCAT®3-Control Vector was used as a control in studies of CAT reporter constructs in CV1 and HeLa cells. GST and GST-YY1 fusion proteins were produced using the TNT® T7 Coupled Reticulocyte Lysate System. (0141)

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