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J. Biol. Chem. 280, 28215-28220. Determination of the functionality of common APOA5 polymorphisms. 2005

Talmud, P.J., Palmen, J., Putt, W., Lins, L., and Humphries, S.E.

Notes: The authors investigated common variants of the APOA5 gene that have been associated with differences in plasma triglyceride (TG) levels. PCR fragments containing either the –1131T --> C promoter variant or containing both the –1131T --> C and –3G --> A promoter variants were cloned into the pGEM®-T Vector System. The fragments were subsequently cloned into the pGL3 Basic Vector and transiently transfected into Huh7 and HepG2 cells along with the luciferase control vector, pRL-TK. The cells were lysed 48 hours after transfection and Luciferase activity was measured with the Dual-Luciferase® Reporter Assay System. The function of the 1891T --> C variant in the 3´ UTR was tested the same way; with the exception that site-directed mutagenesis was performed to introduce the T --> C at position 1891 before the fragment was cloned into the pGL3 Basic Vector. The functionality of the Kozak sequence –3A --> G variant was determined by cloning cDNAs into the pGEM®-7Zf Vector. Transcription/translation experiments were performed using the TNT® Quick Coupled Transcription/Translation System and the proteins were labeled using the FluorTect™ GreenLys System. In addition, a primer extension inhibition assay was performed using capped mRNAs generated with the Riboprobe® System –T7 and the Ribo m7G Cap Analog. Ribosome binding reactions were performed using the Rabbit Reticulocyte Lysate System, Nuclease Treated. (3460)

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Mol. Cell 3, 435-445. Hypermutation in pathogenic bacteria: Frequent phase variation in miningococci is a phenotypic trait of a specialized mutator biotype. 1999

Bucci, C., Lavitola, A., Salvatore, P., Del Giudice, L., Massardo, D.R., Bruni, C.B., Alifano, P.

Notes: The pGEM®-7Zf(+) Vector was used for routine subcloning. (2244)

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Proc. Natl. Acad. Sci. USA 96, 10080-10085. Induction of apoptosis by adenovirus E4orf4 protein is specific to transformed cells and requires an interaction with protein phosphatase 2A. 1999

Shtrichman, R., Sharf, R., Barr, H., Dobner, T., Kleinberger, T.

Notes: The pGEM®-7Zf(+) Vector was used for routine subcloning. (0401)

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Proc. Natl. Acad. Sci. USA 96, 1941-1946. Mg-chelatase of tobacco: The role of the subunit CHL D in the chelation step of protoporphyrin IX. 1999

Gräfe, S., Saluz, H.-P., Grimm, B., Hänel, F.

Notes: The pGEM®-7Zf(-) Vector was used for routine subcloning and in vitro translation reactions. (1115)

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J. Clin. Invest. 103, 1729-1735. Mitogen-activated protein kinase inhibits 1,25-dihydroxyvitamin D3-dependent signal transduction by phosphorylating human retinoid X receptor alpha. 1999

Solomon, C., White, J.H., Kremer, R.

Notes: The pGEM®-7Zf(+) Vector was used for routine subcloning. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to asses viability following the growth of HPK1A and HPK1Aras cells in increasing amounts of the compound LG1069. (0351)

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J. Clin. Oncol. 17, 2419. Nerve growth factor expression correlates with perineural invasion and pain in human pancreatic cancer. 1999

Zhu, Z., Friess, H., diMola, F.F., Zimmermann, A., Graber, H.U., Korc, M., Buchler, M.W.

Notes: The pGEM®-7Zf(+) Vector was used for routine subcloning. The resulting plasmid construct was used as a template for in vitro transcription. (0065)

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Genetics 150, 1639-1648. Molecular consequences of Ds insertion into and excision from the helix- loop-helix domain of the maize R gene. 1998

Liu, Y., Wang, L., Kermicle, J.L., Wessler, S.R.

Notes: Poly(A)+ RNA was purified with PolyATtract® mRNA Isolation Systems. Anti-Rabbit IgG AP Conjugate, and BCIP/NBT were used in Western blotting. R transcripts were synthesized from the Lc cDNA in the pGEM-7Z(+) Vector, and were used in in vitro translation by Rabbit Reticulocyte Lysate Systems to be used as a positive control in Western. (0781)

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J. Cell Biol. 142, 403-420. Pex20p of the yeast Yarrowia lipolytica is required for the oligomerization of thiolase in the cytosol and for its targeting to the peroxisome. 1998

Titorenko, V.I., Smith,J.J., Szilard, R.K., Rachubinski, R.A.

Notes: Both the pGEM®-5Zf(+) Vector and the pGEM®-7Zf(+) Vector were used for routine subcloning of restriction fragments of the PEX20 gene in preparation for sequencing. (0269)

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Proc. Natl. Acad. Sci. USA 94(9), 4354-4359. Purification and characterization of a human RNA adenosine deaminase for glutamate receptor B pre-mRNA editing. 1997

Yang, J.H., Sklar, P., Axel, R. and Maniatis, T.

Notes: Poly A+ RNA was isolated from HeLa cell total RNA using the PolyATtract® mRNA Isolation System and used for RT-PCR. (1699)

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