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Science 285, 248–251. HMG-1 as a late mediator of endotixin lethality in mice. 1999

Wang, H., Bloom, O., Zhang, M., Vishnubhakat, J.M., Ombrellino, M., Che, J., Frazier, A., Yang, H., Ivanova, S., Borovikova, L., Manogue, K.R., Faist, E., Abraham, E., Andersson, J., Andersson, U., Molina, P.E., Abumrad, N.N., Sama, A. and Tracey, K.J.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from RAW 264.7 macrophages treated for various times with endotoxin. The isolated RNA was used for RT-PCR analysis of High Mobility Group-1 gene expression as well as β-actin. RT-PCR was performed with the Access RT-PCR System. (0198)

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Biochem. J. 341, 453–460. Metabolic characteristics of a human hepatoma cell line stably transfected with hormone-sensitive lipase 1999

Pease, R.J., Wiggins, D., Saggerson, E.D., Tree, J., Gibbons, G.F.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from transfected and untransfected HepG2 cells. The isolated RNA was used for Northern blot analysis. (0554)

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J. Bacteriol. 181, 3310–3316. NahY, a Catabolic Plasmid-Encoded Receptor Required for Chemotaxis of Pseudomonas putida to the Aromatic Hydrocarbon Naphthalene. 1999

Grimm, A.C. and Harwood, C.S.

Notes: Total RNA was isolated from Pseudomonas putida using the SV Total RNA Isolation System. RT-PCR using the Access RT-PCR System allowed the authors to characterize the transcriptional organization of several genes involved in naphthalene degradation. (2307)

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J. Virol. 73, 1695–1698. Nonstructural C protein is required for efficient measles virus replication in human peripheral blood cells. 1999

Escoffier, C., Manie, S., Vincent, S., Muller, C.P., Billeter, M., Gerlier, D.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from measles virus-infected and -uninfected human PBMC. The isolated RNA was used for dot blot hybridization to specifically quantitate viral mRNA and the viral RNA genome. (1177)

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J. Biol. Chem. 274, 21830–21839. Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase from mung beans. 1999

Leiter, H., Mucha, J., Staudacher, E., Grimm, R., Glössl, J. and Altmann, F.

Notes: Total RNA was isolated from 3-day-old mung bean hypocotyls using the SV Total RNA Isolation System. RT for RT-PCR was performed with the Reverse Transcription System. (0825)

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Science 284, 502–504. Regulation of mammalian circadian behavior by non-rod, non-cone, ocular photoreceptors. 1999

Freedman, M.S., Lucas, R.J., Soni, B., von Schantz, M., Muñoz, M., David-Gray, Z. and Foster, R.

Notes: Total RNA was isolated from 5–10 mouse retinas with the SV Total RNA Isolation System. The isolated RNA was used for Northern analysis and RT-PCR. (1174)

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Science 284, 505–507. Regulation of the mammalian pineal by non-rod, non-cone, ocular photoreceptors. 1999

Lucas, R.J., Freedman, M.S., Muñoz, M., Garcia-Fernández, J.-M. and Foster, R.G.

Notes: Total RNA was isolated from mouse eyes using the SV Total RNA Isolation System. Four to six eyes produced sufficient quantities of RNA for Northern analysis (at least three blots at 5µg per lane) and RT-PCR. (0749)

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J. Invest. Dermatol. 113, 711–719. Regulation of the melanoma cell adhesion molecule gene in melanoma: Modulation of mRNA synthesis by cyclic adenosine monophosphate, phorbol ester, and stem cell factor/c-Kit signaling. 1999

Karlen, S. and Braathen, L.R.

Notes: The SV Total RNA Isolation System was used to extract total RNA from 106 SK-Mel2 human melanoma cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. The results were used for semi-quantitative analysis. (0959)

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J. Biol. Chem. 274, 6476–6482. Regulatory sequences of the mouse villin gene that efficiently drive transgenic expression in immature and differentiated epithelial cells of small and large intestines. 1999

Pinto, D., Robine, S., Jaisser, F., El Marjou, F.E. and Louvard, D.

Notes: The authors used the SV Total RNA Isolation System to isolate RNA from small intestine, colon, kidney, stomach, liver, heart, lung, thymus, brain, spleen and muscle from transgenic mice. (0535)

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Am. J. Hum. Genet. 65, 1547–1560. The molecular basis of Sjogren-Larsson syndrome: Mutation analysis of the fatty aldehyde dehydrogenase gene. 1999

Rizzo, W. B., Carney, G. and Lin, Z.

Notes: Genomic DNA was purified from blood or cultured fibroblasts, by means of the Wizard® Genomic DNA Purification Kit. Total RNA was isolated from cultured fibroblasts by use of the SV Total RNA Isolation System, and it was amplified by Access RT-PCR System by use of exonic primers. An expression vector containing an S-tag attached to the 5´ end of the FALDH cDNA was constructed using pCI-neo Mammalian Expression Vector. (0482)

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J. Invest. Dermatol. 111, 1053–1057. Expression of interleukin-12 is increased in psoriatic skin. 1998

Yawalker, N., Karlen, S., Hunger, R., Brand, C.U. and Braathen, L.R.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from normal skin, psoriatic skin, H-128 small cell lung carcinoma cells and peripheral blood mononuclear cells. The isolated RNA was used for RT-PCR performed with the Access RT-PCR System. (0107)

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Proc. Natl. Acad. Sci. USA 95, 8286–8291. Mutagenesis associated with nitric oxide production in macrophages. 1998

Zhuang, J.C., Lin, C., Lin, D. and Wogan, G.N.

Notes: Total RNA was isolated from ~3 × 106 RAW264.7 macrophages using the SV Total RNA Isolation System. The amount of recovered RNA was adjusted to 500ng/µl and stored at –20°C. One microgram of the RNA was used for RT-PCR analysis. (0066)

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J. Biol. Chem. 273, 19378–19382. Restoration of beta1A integrins is required for lysophosphatidic acid-induced migration of beta1-null mouse fibroblastic cells 1998

Sakai, T., Peyruchaud, O., Fässler, R. and Mosher, D.F.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from mouse fibroblasts. The isolated RNA was used for RT-PCR analysis with the Access RT-PCR System. (0474)

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