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Eur. J. Pharmacol. 266(3), R1-3. Expression of NMDAR1-1a (N598Q)/NMDAR2A receptors results in decreased cell mortality. 1994

Cik, M., Chazot, P.L. and Stephenson, F.A.

Notes: A short rapid communication where the authors use the CytoTox® 96 Non-Radioactive Cytotoxicity Assay to measure cell death. HEK 293 cells were transfected with various plasmids containing the respective NMDA receptor subunit cDNAs by the calcium phosphate method. (1738)

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Cell 77(6), 817-27. Hydrogen peroxide mediates amyloid beta protein toxicity. 1994

Behl, C., Davis, J.B., Lesley, R. and Schubert, D.

Notes: Using a PC-12 clone, the authors assessed Beta toxicity by three assays; MTT reduction, LDH release (CytoTox® 96 Non-Radioactive Cytotoxicity Assay) and visual counting with trypan blue. Research focuses on antioxidant activity on the amyloid beta protein which accumulates in the central nervous system in Alzheimer's disease. Please note that these authors did not use the CytoTox 96® Assay for cell-mediated cytotoxicity, however it is an interesting article looking at mechanisms of toxicity. (1736)

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Eur. J. Immunol. 23(12), 3217-23. Carrier-mediated uptake and presentation of a major histocompatibility complex class I-restricted peptide. 1993

Brander, C., Wyss-Coray, T., Mauri, D., Bettens, F. and Pichler, W.J.

Notes: The authors compared the CytoTox® 96 Non-Radioactive Cytotoxicity Assay to 51Cr and found the assays were equally sensitive and gave identical results. All cytotoxicity data shown are based on LDH release measurements. EBV-transformed B cell lines, flu peptide specific effector cells also were used (all human lines and cells). (1737)

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J. Neurosci. Methods 20(1), 83-90. Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay. 1987

Koh, J.Y. and Choi, D.W.

Notes: The authors found LDH release to be a simple means to assess glutamate mediated central neuronal cell injury of cortical cells (containing both neuronal and glial elements from fetal mice). They measured LDH by following NADH at 340nm (i.e., non-colorimetric) and found that LDH is extremely stable, even without serum, in culture medium when stored at 37°C. The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to measure LDH release. (1746)

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