PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer.
Li, J., Yen, C., Liaw, D., Podsypanina, K., Bose, S., Wang, S.I., Puc, J., Miliaresis, C., Rodgers, L., McCombie, R., Bigner, S.H., Giovanella, B.C., Ittmann, M., Tycko, B., Hibshoosh, H., Wigler, M.H., Parsons, R.
Notes: To search for mutations in the PTEN gene, PTT was performed using the TNT® T7 Coupled Reticulocyte Lysate System, on 20 breast, 6 glioblastoma and 2 prostate tumor cell lines. Two truncating mutations were identified in PTEN in the breast samples, three in the six glioblastoma lines and in both of the prostate cancer cell lines. One of the mutations in the prostate cancer cell line affected translation initiation. It is believe that more mutations may exist in these lines but were not detected due to the limits of PTT and the fact that the entire coding region was not analyzed. (0794)
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