Arthur, J.S., Elce, J.S., Hegadorn, C., Williams, K. and Greer, P.A.
Notes: The DeadEnd™ Colorimetric Apoptosis Detection System (DeadEnd™ Colorimetric TUNEL System) was used to look at apoptosis in rat embryo sections. Embryos were fixed overnight in 4% paraformaldehyde in phosphate-buffered saline (pH 7.2), dehydrated through graded ethanol, and embedded in paraffin wax. Serial cross or sagittal 7µm sections were dewaxed in xylene, rehydrated through graded ethanol, and stained with hematoxylin and eosin, then the TUNEL assay was carried out using the DeadEnd™ System protocol. (2146)