Bridges, J.P., Wert, S.E., Nogee, L.M. and Weaver, T.E.
Notes: A minimal promoter from the gene encoding BiP was cloned into the pGL3-Basic Vector. The resulting construct was transfected into HEK293 cells and, 48 hours post transfection, cell lysates were prepared using Glo Lysis Buffer. The Bright-Glo™ Luciferase Assay System was then used to assess levels of luciferase expression. As a transfection control, cells were co-transfected with the pSV-β-Galactosidase Control Vector. (3229)