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GoTaq® Probe qPCR: A Real-Time PCR Master Mix Optimized for Hydrolysis Probe Assays Poster

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Abstract

Nadine Nassif, M.S., Gabriela Saldanha, M.S., Doug Storts, Ph.D.

The GoTaq® Probe qPCR Master Mix is a ready-to-use, 2X master mix for qPCR using hydrolysis probe detection, and is designed for sensitive detection and quantification over a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors. Rapid hot-start activation and processive enzymes makes these mixes compatible with any real-time PCR. The GoTaq® Probe 2-Step RT-qPCR facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method. The GoScript™ Reverse Transcription System enables efficient synthesis of first-strand cDNA which may be added directly to downstream qPCR reactions. The GoTaq® Probe 1-Step RT-qPCR is optimized for detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions.

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  • Part# PS228
  • Printed in USA.