Purification of Amplifiable Nucleic Acid from Oil Palm and Rice Leaves and Seeds Using a Small Benchtop Automated Instrument Poster
Part # PS237
Brad Hook1, Samantha Lewis1, Eric Ting2, Youngryul Jung3, Laurence Delaurière4, Elisa Ruiz4, Céline Ménager4, Chris Moreland1 and Trista Schagat1
1Promega Corporation, Madison, WI; 2Promega PTE LTD, Singapore; 3Promega Korea LTD, Seoul, Korea; 4PETAL, Charbonnieres, France
We evaluated a novel cellulose-based paramagnetic particle technology to purify NA from Elaeis guineensis (oil palm) and Oryza sativa (rice) plant leaf and seed lysates in an automated format using the Maxwell® 16 System. Extracted NA was evaluated for yield and amplifiability in qPCR and RT-qPCR. We also tested DNA and RNA extraction from a variety of fruit and vegetable leaves including Malus domestica (apple), Vitis vinifera (grape), Citrus lemon (lemon), Solenum tuberosum (potato), and Solanum lycopersicum (tomato), and found all to yield amplifiable NA. These data support the broad utility of the novel cellulose-based technology and Maxwell® 16 System for automated plant NA purification.
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