Investigating Key Steps in PROTAC™-Mediated Cellular Protein Degradation
Proteolysis targeting chimeras or PROTACs induce selective protein degradation by chemically linking a protein target with an the E3 ubiquitin ligase
component, initiating degradation of the protein target via the ubiquitin-proteosome system (UPS). Interrogating the key steps required for effective PROTAC™-mediated target protein degradation remains a challenge, particularly in cells.
NanoLuc® technologies provide a platform of live-cell assays that can be used to measure real-time degradation kinetics and the key cellular interactions along the UPS that mediate the degradation process. You can even study endogenous protein response when HiBiT is inserted using CRISPR gene editing.