NanoLuc™: A Smaller, Brighter and More Versatile Luciferase Reporter
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Brock received his Ph.D. from the Dept. of Biochemistry and received his B.S. in the Dept. of Chemical and Biological Engineering both at University of Wisconsin-Madison. Brock has been working at Promega for 7 years. Within his time at Promega he has been a group leader in the development of GloSensor Technology. Over the last two years, his group has helped develop and bring NanoLuc Luciferase to market.
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- Original Webinar Date: Tuesday, June 12, 2012
NanoLuc™ (Nluc) is an ATP-independent luciferase that utilizes a novel substrate, furimazine, to produce high intensity, glow-type luminescence. The enzyme, evolved from a deep-sea shrimp, is much brighter than firefly luciferase (FLuc) and provides superior sensitivity as a genetic reporter. It exhibits greater physical stability, which reduces compound interference in highthroughput screening (HTS). NanoLuc™ may be configured for intracellular or secreted reporter formats, and being less than 1/3 the size of FLuc, it is well suited for experiments requiring small reporters (e.g., viral genomes, protein fusions, etc.).