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Protein:Protein Interactions

Protein-protein interaction measured using BRET

Interactions between proteins modulate a large number of biological processes—from fleeting interactions during the transduction of a signaling pathway to the assembly of large multiprotein complexes. NanoLuc® Luciferase provides two new ways to understand these interactions, identify the partners involved and decipher their biological significance.

The extreme brightness and small size of NanoLuc® Luciferase make it possible to label proteins with less risk of interfering with natural function, and allow detection of interactions in vivo under natural conditions. NanoLuc®-based assays utilizing complementation and BRET-based detection are available.

NanoBiT® Reporter: Complementation-Based Detection of Protein Interactions

NanoLuc® Binary Technology (NanoBiT) is a two-subunit system based on NanoLuc® luciferase that can be applied to the intracellular detection of protein:protein interactions (PPIs) in live cells. The NanoBiT® system is composed of two small subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 1.3kDA), that are expressed as fusions to target proteins of interest. The LgBiT and SmBiT subunits have been independently optimized for stability and minimal self-association. Interaction of the target proteins facilitates subunit complementation to give a bright, luminescent enzyme.

Explore NanoBiT® Assays

NanoBRET™ Assays: BRET-Based Detection of Protein Interactions

The study of PPIs with BRET relies on energy transfer from luciferase (BRET donor) attached to one interacting partner, to a fluorescent BRET acceptor tagged to another interacting partner. The transfer of energy is moderated by the proximity of the two partners. The NanoBRET™ assay is a substantially improved BRET assay that uses NanoLuc® luciferase as the energy donor and HaloTag® Protein Labeled with the NanoBRET™ 618 fluorophore as the energy acceptor. The bright, blue-shifted signal from the NanoLuc® donor combined with the far-red-shifted HaloTag® acceptor create an assay with optimal spectral overlap, increased signal, and lower background compared to conventional BRET assays.

Design your own assay or choose from a large selection of Prebuilt NanoBRET PPI Assays targeting: Bromodomain other Epigenetic Proteins, Transcriptional Proteins, Signaling Proteins and Kinase Assays, and Membrane Protein Assays.

Explore NanoBRET Assays

Explore NanoLuc® Luciferase

Discover NanoLuc® Luciferase

The small size and bright luminescence of NanoLuc® Luciferase bring exquisite sensitivity to reporter assays and new possibilities to diverse bioluminescence applications.

Discover NanoLuc® Luciferase
Target Engagement

Quantify the interaction between a molecule and a protein in real time, live cell assays.

Target Engagement
Biosensors

BRET-based biosensors to detect signaling events inside and outside of the cell.

Biosensors
Protein Stability

Monitor changes in intracellular protein abundance with NanoLuc® fusion proteins.

Protein Stability
Gene Regulation & Cell Signaling

Greater sensitivity and versatility for traditional gene regulation assays.

Gene Regulation & Cell Signaling
Imaging

Sensitive, bright bioluminescent imaging in cells and whole animals.

Imaging
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