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Localization of proteins in cells detected with NanoLuc Luciferase

Sensitive, Bright Bioluminescent Imaging: No Excitation Required

Whole Animal Imaging

Bioluminescent imaging of cells and molecular processes in whole animals is important for studying normal physiology, monitoring disease progress or understanding response to therapy. The bright NanoLuc® reporter provides high sensitivity and low background when imaged in superficial mouse tissues and has also been used successfully to image events in deeper tissues. In addition, substrate specificity allows NanoLuc® and firefly luciferases to be used together for dual luciferase molecular imaging studies. Applications range from quantifying changes in tumor growth to visualizing viral replication and spread using engineered NanoLuc® reporter viruses.

Read an Article Using NanoLuc Technology For Live Animal Imaging

Cell-based Imaging

Control of subcellular localization is an important mechanism for regulating the function and signaling activity of many proteins. For example, protein translocation from the cytosol to the nucleus or protein recruitment to the plasma membrane can be key events in signaling pathway activation. Bioluminescent imaging (BLI) can be used to monitor subcellular protein localization, allowing direct visualization of protein dynamics in living cells without the need for repeated sample excitation.

Video showing Translocation of a NanoLuc® Fusion Protein

Monitoring translocation of a NanoLuc® Fusion Protein using Bioluminescence Imaging (BLI). HEK293 cells expressing a Protein Kinase C (PKC)-NanoLuc luciferase fusion protein were measured for 20 minutes following PMA treatment and application of furimazine substrate. BLI was performed on an Olympus LV200 Bioluminescence Imager.

The NanoLuc® reporter is well-suited for use as a protein tag in BLI studies. The extreme brightness means that exposure times can be reduced to only a few seconds compared to the minutes required for other luminescent reporter proteins. Also, the small size of NanoLuc® makes it less likely to perturb the normal biology or functionality of the fusion partner.

Step 1: Choose a Vector to Create NanoLuc® Fusion Protein

Choose a NanoLuc® Fusion Vector. We offer a range of vectors designed for simple generation of NanoLuc® luciferase N- or C-terminal fusion proteins with CMV-driven expression.

View NanoLuc® Fusion Vectors

Additional NanoLuc® Vectors are available in a variety of configurations for additional cloning options.

View Additional NanoLuc® Vectors Request NanoLuc® Vector Sample

Step 2: Choose a Detection Reagent

For standard assays, the Nano-Glo® Luciferase Assay provides a simple, "add-and-read" method for measuring NanoLuc® fusion protein abundance.

View Nano-Glo® Luciferase Assay System

Long-term live cell detection of NanoLuc® luciferase is possible with new Nano-Glo® Live Cell Reporter Substrates under development. Please inquire using the link below if you are interested in testing these substrates.

Please Inquire

Step 3: Image NanoLuc® Fusion Protein

The Olympus LV200 Bioluminescence Imaging System has been successfully used for BLI of NanoLuc® fusion proteins.

Go to Olympus Website

Explore NanoLuc® Luciferase

Discover NanoLuc® Luciferase

The small size and bright luminescence of NanoLuc® Luciferase bring exquisite sensitivity to reporter assays and new possibilities to diverse bioluminescence applications.

Discover NanoLuc® Luciferase
Protein:Protein Interactions

New ways to understand protein interactions, identify the partners involved, and decipher their biological significance.

Protein:Protein Interactions
Target Engagement

Quantify the interaction between a molecule and a protein in real time, live cell assays.

Target Engagement

BRET-based biosensors to detect signaling events inside and outside of the cell.

Protein Stability

Monitor changes in intracellular protein abundance with NanoLuc® fusion proteins.

Protein Stability
Gene Regulation & Cell Signaling

Greater sensitivity and versatility for traditional gene regulation assays.

Gene Regulation & Cell Signaling