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Gene Regulation and Cell Signaling

Gene Regulation study using NanoLuc Luciferase

Using NanoLuc® Luciferase in Reporter Gene Assays

Reporter Gene Assays are powerful tools for studying gene regulation and serving as indicators of specific cellular signals. By cloning a promoter or 3´-UTR of interest into a plasmid containing the NanoLuc® Luciferase reporter gene, it is possible to study elements in these DNA sequences that regulate gene expression. Alternatively, by inserting defined elements known to bind to different classes of transcription factors or enhancers into these vectors, assays measuring cell signaling pathways that activate these factors can be built.

Greater Sensitivity, Small Size and Versatile Reporter Configurations

  • Sensitive Detection at Low Expression Levels: The high-intensity luminescence of the NanoLuc® enzyme combined with low autoluminescence of the furimazine substrate allows sensitive detection of low levels of luciferase expression, even in difficult experimental systems such as primary cells with poor transfection or expression from endogenous promoters.
  • Limited Risk of Disrupting Natural Biology: Because NanoLuc® Luciferase is small (19kDa; 516bp), it can be used in applications where gene size is critical, such as viral packaging.
  • Destabilized and Secreted Forms Available: NanoLuc® luciferase is also available in destabilized form for rapid response to changes in gene expression and in secreted form for extracellular detection from live samples.

Step 1: Choose a Reporter Vector to Express NanoLuc® Luciferase

Many NanoLuc® Genetic Reporter Vectors are available for easy cloning of promoters or putative binding sites.

View NanoLuc® Genetic Reporter Vectors Request NanoLuc® Vector Sample

Pre-designed response element and promoter-containing NanoLuc® Signaling Pathway Vectors are available for a variety of signaling pathways from Promega Custom Assay Services.

Vectors available from Custom Assay Services

Step 2: Choose a Detection Reagent

For standard assays, the Nano-Glo® Luciferase Assay provides a simple, "add-and-read" method for measuring NanoLuc® fusion protein abundance.

View Nano-Glo® Luciferase Assay System

The NanoGlo® Dual Luciferase Reporter Assay allows you to detect the activities of firefly and NanoLuc luciferase in a single, convenient format. The improved firefly chemistry coupled with the sensitivity of NanoLuc luciferase make this the most sensitive, most powerful dual-reporter assay available.

  • Greater Sensitivity at Low Expression Levels
  • Your Choice of Two Primary Reporters-use NanoLuc, Firefly or both as dynamic reporters
  • Better Control, More Reliable Data
Learn more about NanoDLR

Long-term live cell detection of NanoLuc® luciferase is possible with new Nano-Glo® Live Cell Reporter Substrates under development. Please inquire using the link below if you are interested in testing these substrates.

Please Inquire
GloMax Discover Instrument

The Nano-Glo® assay is detected with a microplate luminometer, such as the GloMax® Discover. Learn more about GloMax® Discover here.

Explore NanoLuc® Luciferase

Discover NanoLuc® Luciferase

The small size and bright luminescence of NanoLuc® Luciferase bring exquisite sensitivity to reporter assays and new possibilities to diverse bioluminescence applications.

Discover NanoLuc® Luciferase
Protein:Protein Interactions

New ways to understand protein interactions, identify the partners involved, and decipher their biological significance.

Protein:Protein Interactions
Target Engagement

Quantify the interaction between a molecule and a protein in real time, live cell assays.

Target Engagement
Biosensors

BRET-based biosensors to detect signaling events inside and outside of the cell.

Biosensors
Protein Stability

Monitor changes in intracellular protein abundance with NanoLuc® fusion proteins.

Protein Stability
Imaging

Sensitive, bright bioluminescent imaging in cells and whole animals.

Imaging