Investigating Key Stages in PROTAC™-Mediated Cellular Protein Degradation
Although methods exist to determine target protein levels, directing the target protein to the proteasome with PROTACs is a multistep process. Interrogating these key steps required for effective PROTAC™-mediated target protein degradation remains a challenge, particularly in cells.
NanoLuc® technologies provide a platform of live-cell assays that can be used to measure real-time degradation kinetics and the key cellular interactions along the UPS that mediate the degradation process. You can even study endogenous protein response when HiBiT is inserted using CRISPR gene editing.