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Imaging

NanoLuc® Luciferase provides a tool for sensitive, bright bioluminescent imaging with low background and no excitation required.

Whole Animal Imaging

Imaging Live Animals with NanoLuc® Technology

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Imaging of luciferase activity in live mice.

 Bioluminescent imaging of cells and molecular processes in whole animals provides important insights when studying normal physiology, monitoring disease progress or understanding response to therapy. The bright NanoLuc® reporter provides high sensitivity and low background when imaged in superficial mouse tissues, and has also been used successfully to image events in deeper tissues.

Substrate specificity allows NanoLuc® and firefly luciferases to be used together for dual luciferase molecular imaging studies, as described in the article below. Applications range from quantifying changes in tumor growth to visualizing viral replication and spread using engineered NanoLuc® reporter viruses.

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In Vivo Imaging with NanoLuc® BRET Reporters

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NanoLuc®-based BRET reporters are also being used by researchers to develop in vivo imaging strategies. These techniques utilize the bright NanoLuc® signal to excite fluorescent acceptor proteins, creating enhanced deep tissue imaging solutions.

Read this blog to learn about how two researchers have used this approach.

Read the Blog

Bioluminescent Imaging in Live Cells

 Control of subcellular localization is an important mechanism for regulating the function and signaling activity of many proteins. For example, protein translocation from the cytosol to the nucleus or protein recruitment to the plasma membrane can be key events in signaling pathway activation. Bioluminescent imaging (BLI) can be used to monitor subcellular protein localization, allowing direct visualization of protein dynamics in living cells without the need for repeated sample excitation.

The NanoLuc® reporter is well-suited for use as a protein tag in BLI studies. The extreme brightness means that exposure times can be reduced to only a few seconds, compared to the minutes required for other luminescent reporter proteins. Also, the small size of NanoLuc® makes it less likely to perturb the normal biology or functionality of the fusion partner. New Nano-Glo® Extended Live Cell Substrates provide options for long-term imaging studies.

Monitoring translocation of a NanoLuc® Fusion Protein using Bioluminescence Imaging (BLI). HEK293 cells expressing a Protein Kinase C (PKC)-NanoLuc luciferase fusion protein were measured for 20 minutes following PMA treatment and application of furimazine substrate. BLI was performed on an Olympus® LV200 Bioluminescence Imager.

Interested in receiving updates on new developments for NanoLuc® imaging? Contact Us