We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Detecting Protein:DNA Interactions: ChIP without Antibodies

In the HaloCHIP™ method, a HaloTag® fusion protein is expressed either transiently or stably in mammalian cells and crosslinked complexes are directly captured from the cellular lysate via covalent binding to a HaloTag®-specific resin. The covalent linkage allows for extensive washing to remove non-specific proteins and DNA, before reversal of the crosslinks to release the bound DNA fragments.

  • Simple, 1-day ChIP protocol, without antibodies.
  • Low Background. HaloTag® covalent capture allows stringent washing compared to antibody-dependent ChIP.
  • Savings compared to conventional ChIP kits, which require the purchase of an untested antibody.

Detecting Protein:DNA Interaction with the HaloCHIP™ System

Rapid Protocol Saves Time Compared to Conventional ChIP Assays

9697MB
9697MC

All the Results - Without Searching for an Antibody!

Similar fold enrichment pull-down results were obtained using HaloCHIP™ to pull-down CREB:DNA partners as compared to conventional CREB ChIP.

CREP HaloChIP versus CREB ChIP versus Untransfected

qPCR Data. Conventional (antibody-dependent) ChIP was compared to HaloCHIP for CREB:DNA binding sequences. No significant differences in enrichment patterns were found when comparing known CREB-binding sites: Fos, Jun, and p27.

HaloCHIP™ Captures True Protein:DNA Interactions

There is excellent reproducibility in binding between HaloCHIP™ and conventional antibody-dependent ChIP assays.

CREB ChIP-chip enrichment versus CREB HaloChIP enrichment CREB ChIP-chip enrichment versus CREB Halo-chip enrichment

ChIP-chip data. High-resolution analysis demonstrates nearly identical promoter enrichment profiles for CREB binding when comparing CREB-HaloCHIP™ to CREB-ChIP.

HaloTag® Pull-Down System in the Literature

Hartzell, D.D. et al. (2009) functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays.
BMC Genomics., 2009; 10: 497.
Read more HaloCHIP™ citations