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Real-Time Cytotoxicity Analysis Using the CellTox™ Green Assay Poster

Part # PS179

Abstract

Tracy Worzella1, Andrew Niles1, Thomas Hengstl2, Michael Fejtl2, Christian Oberdanner2, and Jessica Merlino3
1Promega Corporation, Madison WI; 2Tecan Austria GmbH; 3Tecan Group Ltd., Männedorf, Switzerland

In vitro cytotoxicity is largely influenced by test article concentration and the exposure period with cells. The diversity of kinetic response can complicate conventional cytotoxicity endpoint assay determinations because most assay reagents are formulated to measure enzymatic biomarkers that are susceptible to time-dependent decay.

Here we demonstrate the use of a non-activity-based cytotoxicity probe, CellTox™ Green Dye, that can be added at the beginning of the experiment and employed in real time for the kinetic determination of cytotoxicity. We used the Tecan HP D300 Digital Dispenser for non-contact dispensing of test compound, and the Tecan Infinite® 200 PRO with GCM™ for kinetic measurement of cytotoxicity over 72 hours.

Printed in USA.