We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Quantitating DNA from FFPE tissue: Superior sensitivity of the QuantiFluor™ dsDNA...

Quantitating DNA from FFPE tissue: Superior sensitivity of the QuantiFluor™ dsDNA System versus the NanoDrop Spectrophotometer Poster

SEARCH POSTERS

Abstract

Accurate quantitation of DNA concentration is critical for many applications. Traditional spectrophotometric assays cannot determine DNA concentrations below 2µg/ml; however, many isolated DNA samples have concentrations well below this level. The QuantiFluor™ dsDNA System (Cat# E2670) is a fast, easy and sensitive method for determining low DNA concentrations.

Formalin fixation and paraffin embedding is the most commonly used method worldwide for tissue storage. The Maxwell®16 FFPE Tissue LEV DNA Purification Kit (Cat# AS1130) is specifically designed for optimal purification of DNA from one to ten (5µM) sections of FFPE tissue samples. To help minimize PCR inhibitor carryover, small amounts of the DNA-binding paramagnetic particles are used, and as a result the DNA-binding capacity of the system is limited to a few hundred nanograms of pure DNA suitable for amplification applications including qPCR. With such low levels of DNA, an accurate and sensitive method is required for DNA concentration determination.

Double-stranded DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue samples using the Maxwell® 16 FFPE Tissue LEV DNA Purification Kit (Cat# AS1130) was quantitated using both the Quantifluor™ dsDNA System and NanoDrop spectrophotometer. The NanoDrop was only able to determine concentrations for samples above 2ng/µl but Quantifluor™ accurately determined concentrations for all samples.

Document Icon Download the poster PDF (347kb)

  • Part# PS122
  • Printed in USA 3/11.
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom