We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Proteasomal Activity in Skeletal Muscle: A Matter of Assay Design, Muscle Type and Age...

Proteasomal Activity in Skeletal Muscle: A Matter of Assay Design, Muscle Type and Age Scientific Poster

SEARCH POSTERS

Abstract

The ubiquitin proteosome system (UPS) is a major non-lysosomal protein degradation machinery of the cell, which cleaves regulatory, misfolded and damaged proteins into small peptides. The ATP-dependent UPS represents a cascade of enzymatic factors, ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2), and ubiquitin protein ligases (E3), which are associated with the 26S proteasome and give rise to a large macromolecular complex. The barrel-shaped conventional 26S proteasomal structure is composed of one or two regulatory cap domains (19S subunits) and a proteolytically active central core domain (20S subunit), which harbors the three main catalytic activities, the chymotrypsin-like, trypsin-like and peptidyl-glytamyl-like (caspase-like) activities.

In the present study we established and validated a luminescence-based proteasomal activity assay applicable to milligram quantities (typically 5 mg) of skeletal muscle tissue. Special emphasis of our work was put on the influence of tissue preparation and storage, the amount of total protein extract yielding in a stable luminescent signal with optimal signal-to-noise ratio, the usage of different substrates in conjunction with a specific and irreversible proteasomal inhibitor, the difference in the use of individual muscle groups, and the effect of ageing.

Document Icon Download the poster PDF (321kb)

  • Part# PS118
  • Printed in USA.
Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom