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Combining On-Bead Conjugation and MS Analysis After IdeS Digestion Simplifies the Production and Characterization of ADCs

Part # PS222

Abstract

Chris Hosfield1, Philip Compton2, Becky Godat1, Sergei Saveliev1, Archer Smith2, Nidhi Nath1, Paul Thomas2, Neil L. Kelleher2, Kevin Cook1, Michael Rosenblatt1 and Marjeta Urh1
1Promega Corporation, Madison, WI; 2Proteomics Center of Excellence, Northwestern University, Evanston, Il

Magnetic particles offer an efficient and scalable approach for conjugating small molecules to antibodies as demonstrated by conjugation of AlexaFluor to Rituximab.

Printed in USA.