Nucleic Acid Purification From Plant Tissues for Viral Detection

Part # PS285

Abstract

Samantha Lewis¹, Laurence Delaurière², Becky Carroll¹, Elisa Ruiz², Ellyn Lepinski¹, Giuseppe Durante³, Caroline Chatillon⁴, Doug Wieczorek¹,Céline Ménager², Eric B. Vincent¹ and Trista Schagat^1
1Promega Corporation, 2800 Woods Hollow, Madison WI, USA 53711, ²Promega Europe Training and Applications Laboratory (PETAL), 24 Chemin des Verrières, Charbonnières-les-Bains, France 69260, ³International Plant Analysis and Diagnostics srl., Loc. Cascina Codazza, Via Einstein, Italy 26900, ⁴Qualiplante, Cap Alpha, Avenue de l'Europe, Clapiers, France 34830.

The diverse range of plant species and tissue types found in nature present a unique set of obstacles for nucleic acid isolation from botanical samples. In addition, the carry-over of substances present in certain plants can interfere with quantitation and downstream amplifications. These difficulties highlight the need for robust, reliable chemistries for molecular biology studies with plants. We present several solutions to purify and amplify nucleic acid from a variety of plant types and tissues with a focus on agriculturally relevant applications.

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