We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Monitoring Differential Expression in Stem Cells Scientific Poster

SEARCH POSTERS

Abstract

Nadine Nassif1, Nathan Greve1, Soshana Svendsen1, Clive Svendsen2 and Douglas R. Storts1
1Promega Corporation, Madison, WI
2University of Wisconsin-Madison

Stem cell differentiation is routinely monitored by measuring gene expression using real-time reverse transcription PCR. The Plexor® Differential Expression System is a novel, real-time PCR system for the detection and relative quantitation of RNA expression levels, and can be used to monitor stem cell differentiation using multiplexed amplification.

Optimized primer pairs allow users to quantitatively amplify specific transcripts in a two-color duplex reaction. This duplex amplification strategy enables the user to amplify a transcript of interest as well as a reference transcript (GAPDH) in a single reaction.

The first set of optimized primer pairs to be made available will focus on transcripts associated with stem cell pluripotency: NANOG, SOX2, OCT3/4, LIN28, KLF4, and cMYC, each in a duplex with GAPDH. These primers target genes that are expressed at high levels in pluripotent cells (i.e., stem cells and induced pluripotent stem cells [iPS]). In contrast, these genes are expressed at low levels in differentiated cells.

Document Icon Download the poster PDF (179kb)

  • Part# PS097
  • Printed in USA.
Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom