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Development of High Capacity Magnetic Protein A and Protein G Beads for Rapid Antibody...

Development of High Capacity Magnetic Protein A and Protein G Beads for Rapid Antibody Purification from Small Sample Volume Poster

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Abstract

Nidhi Nath, Becky Godat, Brad Hook, Mark Bratz, Trista Schagat, Cris Cowan, Christine Helt, Gregg Cameron, Marjeta Urh
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

We have developed high capacity magnetic Protein A and magnetic Protein G beads by combining two novel technologies:
1. Use of magnetic beads based on macroporous cellulose that provides high surface area for high capacity and low non-specific binding surface for high purity.
2. Covalent and oriented immobilization of Protein A and Protein G on the magnetic bead using HaloTag® Technology.

Oriented immobilization of the proteins has been shown to improve the functionality of the proteins and may contribute to the high capacity of these beads. Magne™ Protein A Beads and Magne™ Protein G Beads allow purification of polyclonal and monoclonal antibodies from serum, ascites fluid and cell media.

Document Icon Download the poster PDF (960kb)

  • Part# PS169
  • Printed in USA.
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