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Considerations for Use of Taq Polymerase in Molecular Diagnostic Assays

Part # PS208

Abstract

Rod Pennington, Terri McDonnell, Jenn Mook, Karen Reece, Letta Steffen, Nadine Nassif, Katharine Hoffmann, Doug Storts
Promega Corporation, Madison, WI

Many molecular diagnostic assays utilize PCR technology to understand and diagnose disease. Taq DNA Polymerase is a commonly used engine to drive PCR. The quality, performance and format of the Taq DNA polymerase enzyme influence the design and performance of a PCR-based molecular diagnostic assay. The ability of the enzyme to perform in multiple PCR applications and multiple Taq DNA polymerase product formats contribute flexibility to the design of the molecular diagnostic assay. Manufacture of the enzyme under GMP yields lot-to-lot consistent enzyme with minimal product contaminants (such as nucleases and nucleic acids) that support a consistently performing molecular diagnostic assay.

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