Product Availability Update

Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Learn more

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction

Part # PS328

Abstract

Terry Riss, Sarah Duellman, Brad Hook, Mark Bratz, Samantha Lewis
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711 

Although the implementation of 3D cell culture models is growing rapidly, there is an unmet need to establish in vitro assay methods to interrogate larger and more complex 3D structures. We report here the results of using a novel small molecule probe that viable cells convert into a substrate for a shrimp-derived luciferase to generate a signal proportional to the number of viable cells. The detection reagent can be incubated with cells and produce a luminescent signal for days while having minimal effect on the biology of the system.

Printed in USA.