Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Characterization of an IgG-Cleaving Protease from Streptococcus equi with Improved Activity Against Mouse IgGs Poster

Part # PS260

Abstract

Chris Hosfield1, Philip Compton2, Luca Fornelli2, Paul Thomas2, Neil L. Kelleher2, Michael Rosenblatt1 and Marjeta Urh1
1Promega Corporation, 2800 Woods Hollow Rd Madison, WI 53711; 2Proteomics Center of Excellence, Northwestern University, Evanston, IL
IdeZ Protease was originally identified in S. equi subsp, zooepidemicus. Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.

Printed in USA.