Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Bioluminescent Metabolite Assays Enable Deeper Characterization of Metabolic Profile and Communication of Cancer and Immune Cells

Part # PS283

Abstract

Michael P. Valley, Natasha Karassina, Donna Leippe, Mary Sobol and Jolanta Vidugiriene
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711

Bioluminescent glucose uptake and metabolite assays provide tools for studying metabolic adaptation of cancer and immune cells. Understanding the metabolic interaction between tumor and immune system can offer new approaches for increasing efficacy of T-cell immunotherapy. We have developed an assay to measure glucose uptake that has the following features:
- Simple & sensitive, applicable to 96-and 384-well format and can be multiplexed with other assays to get more information per well.
- Metabolite assays can be used for measuring changes in culture media, cell lysates or tissues.
- Glucose uptake assay can be used for measuring changes in Glut1-Glut4 activity and produces results equivalent to radioactive approach.

Printed in USA.