Bioluminescent Metabolite Assays Enable Deeper Characterization of Metabolic Profile and Communication of Cancer and Immune Cells
Part # PS283
Michael P. Valley, Natasha Karassina, Donna Leippe, Mary Sobol and Jolanta Vidugiriene
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711
Bioluminescent glucose uptake and metabolite assays provide tools for studying metabolic adaptation of cancer and immune cells. Understanding the metabolic interaction between tumor and immune system can offer new approaches for increasing efficacy of T-cell immunotherapy. We have developed an assay to measure glucose uptake that has the following features:
- Simple & sensitive, applicable to 96-and 384-well format and can be multiplexed with other assays to get more information per well.
- Metabolite assays can be used for measuring changes in culture media, cell lysates or tissues.
- Glucose uptake assay can be used for measuring changes in Glut1-Glut4 activity and produces results equivalent to radioactive approach.
Printed in USA.