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Automation of ADP-Glo Universal Kinase Assay For HTS Screening AND Profiling Applications Poster

Part # PS119

Abstract

Gediminas Vidugiris, Hicham Zegzouti, Jolanta Vidugiriene, Said Goueli
Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711

Growing recognition of kinases as a cornerstone in cell signaling and their increasing importance as drug targets has generated a need for developing technologies that enable rapid characterization of more than 500 kinases present in the genome. Successful adaptation of such technologies on automated liquid handling platforms is an absolute necessity for generating the large amount of data required for characterization of kinases and evaluation of their inhibitors.

Here using Promega’s recently introduced universal ADP-Glo™ kinase assay we show how combining integrated instrumentation allows researchers to efficiently screen and profile compounds for potential identification of new drugs. For this work we have used: automated liquid handling platforms - Beckman Coulter BioRAPTR™ or Tecan TeMo™ workstations with multifunctional plate readers - BMG PHERAstar™ or Tecan Safire2™ or Infinite500™.

The ADP-Glo™ Assay monitors ADP formation, the common product generated in all kinases reactions, providing a universal platform for characterization of all kinase types. Even though the ADP-Glo™ Assay requires coupling the addition of liquid handling steps with wide volume range, as we show here, the combination of advanced chemistry and robust performance on a variety of automated workstations makes the ADP-Glo™ assay attractive for HTS applications.

Printed in USA.