We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Automated Nucleic Acid Purification from ThinPrep PAP Samples for CT/NG Detection...

Automated Nucleic Acid Purification from ThinPrep PAP Samples for CT/NG Detection Scientific Poster

SEARCH POSTERS

Abstract

Samuel Caughron, MD1, Melissa Melby1, Rebecca Roberts2, and Trista Schagat, PhD2
1Yellowstone Pathology Institute, Billings, MT
2Promega Corporation, Madison, WI

ThinPrep® solution is commonly used to preserve cervical swab cells for morphological analysis. We examined automated nucleic acid purification from these samples for molecular assays. DNA isolate by Maxwell® 16 and analyzed for Chlamydia trachomatis and Neisseria gonorrhea (CT/NG) by real-time PCR showed a high correlation to replicate samples assayed by either Roche COBAS® AMPLICOR or Gen-Probe APTIMA Combo 2® Assays in independent laboratories (99% concordance). This same method did not consistently yield DNA of sufficient concentration for Invader®-based Human Papillomavirus (HPV) detection , so we developed a modified extraction method. This method gave 88% concordance to Digene Hybrid Capture II (HC2). These data show Maxwell® 16 can be used for automated nucleic acid purification from ThinPrep® PAP samples for downstream CT/NG and Invader®-based HPV detection.

Document Icon Download the poster PDF (396kb)

  • Part# PS091
  • Printed in USA.
Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom