Automated DNA Purification from Buccal Swabs for qPCR and HLA Typing assays using the Maxwell®16 LEV Blood Kit
Part # PS115
Rebecca Gorshe, MS1, Jennifer Schiller, PhD2, Danielle Meehan, CHT3, and Trista Schagat, PhD1
1Promega Corporation, Madison, WI; 2Blood Center of Wisconsin, Milwaukee, WI; 3University of Rochester Medical Center, Rochester, NY
The Human Leukocyte Antigen (HLA) system is a group of genes that makes up the major histocompatibility complex (MHC). This super locus contains a large number of genes related to immune system function in humans. HLA typing is used to determine whether a tissue or organ donor is a suitable match for a patient. Typically, DNA is extracted from blood or buffy coat and the DNA is analyzed by Sequence Specific Oligonucleotides (SSO) typing or Sequence Specific Priming (SSP) typing. The use of buccal or cheek swabs is an advantageous alternative to a blood draw because of its non-invasiveness and the lack of reliance on the donors white blood cell count. Here we describe an application of the Maxwell®16 LEV Blood DNA Kit (Cat. #AS1290) for the automated extraction of DNA from buccal swab samples for use with SSO and SSP analysis for HLA typing. We also describe this application for compatibility for qPCR assays.
Printed in USA.