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An Automated Solution to Antibody Purifications Scientific Poster

Part # PS182

Abstract

Mark Bratz, Brad Hook, Trista Schagat, Nidhi Nath, Becky Godat
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711. E-mail: mark.bratz@promega.com

We have developed high capacity magnetic Protein A and magnetic Protein G beads by combining two novel technologies.

  1. Use of magnetic beads based on macroporous cellulose that provides high surface area for high capacity and low non-specific binding surface for high purity.
  2. Covalent and oriented immobilization of Protein A and Protein G on the magnetic bead using HaloTag® Technology. Oriented immobilization of the proteins has been shown to improve the functionality of the proteins and may contribute to the high capacity of these beads.

Magne™ Protein A Beads and Magne™ Protein G Beads allow purification of polyclonal and monoclonal antibodies from serum, ascites fluid and cell media.

 

Printed in USA.