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A Novel 6 x 5 Peptide Mixture for Performance Monitoring

Part # PS219

Abstract

Michael M. Rosenblatt1, Ethan Strauss1, Joshua Beri2, Michael Bereman3 and Marjeta Urh1

1Promega Corporation, 2800 Woods Hollow Rd, Madison WI 53711
2Department of Chemistry and 3Department of Biological Sciences, North Carolina State University, Raleigh, NC

Performance monitoring of LC-MS/MS instrumentation continues to be challenging and has yet to be standardized across all MS laboratories. Here we report the development of a novel reference peptide mixture and an accompanying software tool. The mixture reports on LC column performance, MS and MS/MS instrument parameters, including sensitivity and dynamic range. In addition, the software can report parameter history, compare instruments, and display XIC traces. This is the first example of a peptide mixture designed to give a report of all critical LC and MS parameters in a single run.

Printed in USA.