Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Limited Functionality Warning

We are currently updating our network. During this time, certain functionality may be unavailable, including online orders. We apologize for any inconvenience this may cause you.

Please contact Customer Service with any questions or comments.
Phone: (608) 274-4330
Toll-Free Phone: (800) 356-9526
Email: custserv@promega.com
Hours: 7am – 6pm, CST, Monday-Friday

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

A Cell-Based Luciferase Reporter Bioassay for Interleukin-2 and Interleukin-15 Testing

Part # PS349

Abstract

1Richard Moravec, 2David Adle, 1Frank Fan and 1Mei Cong
1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
2Covance Inc., BioCMC,  Greenfield, IN 46140 

IL-2 and IL-15 are still clinically important cytokines as researchers look to improve potency, patient tolerance and response by developing new molecules with sustained and targeted activities. PEGylation, superagonists, immunocomplexes, and immunocytokines are current strategies in clinical development.

We have developed a luciferase reporter bioassay which can be used for the quantitation of both IL-2 and IL-15 using the cytokine’s mechanism of action pathway. The bioassay format is based on thaw-and use cells, eliminating the need to establish and pre-culture traditional IL-2 responsive cells such as CTLL-2.  This format also provides the benefit of convenience, reproducibility, and transferability. Quantitative measurement of IL-2 or IL-15 using this reporter bioassay is complete in less than 7 hours, versus 2–3 days using traditional proliferation assay protocols. We show the bioassay is stability indicating using heat-stressed aldesleukin samples. And finally the cell line demonstrates stability and specificity. 

Printed in USA.