A Bioluminescent Assay Enables Easy Measurement of Glucose Uptake
Part # PS281
Michael P. Valley, Natasha Karassina, and Jolanta Vidugiriene
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711
Abstract # 2107
Glucose uptake is an important pharmacological target. Cancer cells support their high rates of proliferation by overexpressing glucose transporters to increase their rates of glucose uptake. In contrast, the decreased rates of glucose uptake in diabetic fat and muscle cells can lead to hyperglycemia and its inherent deleterious biological effects. Hence, effectors of glucose uptake would be useful for both anticancer therapies and diabetes management.
The standard method for measuring glucose uptake has long been the addition of a radioactive glucose analog (2-deoxyglucose) and measurement of the accumulation of the stable and impermeable phosphorylated derivative, 2-deoxyglucose-6-phosphate (2DG6P). In the interest of transitioning to a safer, non-radioactive assay, we have developed a simple bioluminescent glucose uptake assay that measures the production of NADPH through the oxidation of 2DG6P by glucose-6-phosphate dehydrogenase (G6PDH). This assay is both rapid and convenient and exhibits a larger signal window than comparable fluorescent or colorimetric approaches.
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