Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Normalization and Direct Amplification of Casework Samples

Part # PS293

Abstract

Jeanne M. Bourdeau1, Robert S. McLaren1, Massimiliano Stabile2, Nicola Staiti2, Stella Eugenia Cirillo3, Giampietro Lago2, and Douglas R. Storts1.
1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711;
2Carabinieri RIS Parma, Sez. Biologia, Parma, Italy;
3Università di MilanoBicocca, Milano, Italy

Integration of the prototype Casework Direct Solution Kit into a laboratory workflow scheme provides a rapid, cost-effective means to generate high quality STR profiles from precious, low-abundance samples with minimal hands on time. This kit rapidly generates lysates from casework swabs and fabric cuttings which are compatible with the PowerQuant®System and PowerPlex® STR Systems.
The PowerQuant®System provides valuable workflow information such as the presence/absence of male DNA, degradation, or potential PCR inhibition, as well as normalization for STR reactions. If no inhibition is detected in the PowerQuant®System it is possible to go directly into a PowerPlex®System STR amplification reaction without any DNA purification, while DNA may be purified from lysates containing inhibitorswith Maxwell®16.

Printed in USA.