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Kinase Strips for Routine Creation of Inhibitor Selectivity Profiles: A Novel Approach to Targeted Profiling Poster

Part # PS247


Hicham Zegzouti, Jacquelyn Hennek, Juliano Alves, and Said Goueli
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

We created new kinase profiling systems for simple kinase inhibitor profiling studies based on the luminescent ADP-Glo kinase assay platform. Using the new kinase profiling strips we could easily generate selectivity profiles using small or large kinase panels, as well as identifying compound promiscuity towards members of a single kinase subfamily or different subfamilies of the kinome. With these advantages, the bioluminescent kinase platform can now allow for routine performance of inhibitor profiles in quick, affordable and flexible manner using one assay format for all classes of kinases during the drug discovery process.

Printed in USA.