In-Gel Digestion of Mouse Membrane Protein Extract: Increased Peptide Recovery and Identification of Very Low Abundance Hydrophobic Proteins

Part # PS099

Abstract

Christopher M Adams¹, Allis S. Chien¹, Dan Simpson², Bill Dailey², Sergei Saveliev^2
1Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University, Stanford, CA
²Promega Corporation, Madison, WI

In mammalian proteomes, it is estimated that 6,000–8,000 genes encode for membrane proteins. Yet, large scale proteomic analysis of these same membrane proteins remains a challenge. The hydrophobic nature of membrane proteins most commonly results in poor protein solubility. For in-gel digestion protocols, poor protein solubility for both hydrophobic and hydrophilic peptides results in inefficient digestion and peptide extraction.

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