Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Improved Chemistries for NGS Library Cleanup and Size Selection

Part # PS313


Charles Cowles, PhD, Douglas Horejsh, PhD, Mark Denhart, Curtis Knox, Adam Blatter, Marjeta Urh, PhD
Promega Corporation, 2800 Woods Hollow Rd. Madison, WI 53711

Next Generation Sequencing (NGS) libraries require high quality nucleic acid inputs of varying quantities, concentration, and size depending on the library preparation methods and sequencing platforms used. Regardless of these variations, in most instances a magnetic bead-based chemistry is utilized as a portion of the overall protocol. The steps using magnetic bead chemistries fall into two basic categories of function:
1) Sample cleanup: Removes sequencing adaptors or PCR primers, dNTP’s, enzymes or unwanted buffer formulations.
2) Size Selection: Removes unwanted nucleic acid fragment or library molecules that are above or below a specified size range optimal for the downstream sequencing platform.

By varying the ratio of bead chemistry added to the original volume of DNA in solution, the user can alter the size of DNA captured by the beads or left behind in solution.

Printed in USA.