Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

HaloTag® Technology for Protein Expression, Solubilization and Purification

Part # PS096


Kate Qin Zhao, Rachel Friedman Ohana, Marjeta Urh, Jackie Kinney, John Eckert, Lance Encell and Keith Wood
Promega Corporation, Madison, WI

The HaloTag® protein is engineered from a bacterial dehalogenase (MW 34kDa) to covalently attach to a set of chloroalkane ligands with different functional groups, such as fluorescent dyes, biotin and solid surfaces. It has been successfully applied to live cell imaging, protein interactions and protein immobilization.

Designed for structural compatibility with fusion protein partners, HaloTag Technology has the following advantages:

• Enhancement of target protein expression/solubility as compared to GST, MBP and HIs6Tag in E. coli.

• Covalent immobilization with HaloLink™ Resin for better protein recovery and low nonspecific contaminants.

• Optimized TEV linker for better target protein release.

• In-gel detection and quantification of protein expression levels by 1:1 stoichiometric labeling with fluorescent HaloTag ligands.

Printed in USA.