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A No-Wash, Rapid FcRn Binding Immunoassay to Guide the Design and Development of Antibody Therapeutics

Part # PS357

Abstract

Nidhi Nath, Becky Godat, Rod Flemming and Marjeta Urh
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

Current methods of analyzing FcRn-Ab interactions involve immobilization of FcRn and/or antibody and have been shown to introduce artifacts resulting in irreproducible results. Here we present a solution-based (no-immobilization step) homogeneous (no-wash step) assay for FcRn-Ab interaction using NanoBiT® technology.

Printed in USA.