Compound Interference of CellTiter-Glo® vs PE ATPlite™ 1Step
Part # PS143
Brad Hook and Trista Schagat
Promega Corporation, 2800 Woods Hollow Road, Madison, WI USA 53711
Reducing the number of false positives in a high-throughput screen is a key goal for any researcher trying to minimize downstream efforts. Luciferase-based systems are used widely as reporters for drug screens; however, compounds in a library can inhibit luciferase, resulting in false positives. Ultra-Glo™ rLuciferase, an evolved luciferase from the firefly, Photuris pennsylvanica, is less sensitive to compound inhibition than wild-type firefly luciferase. In this study, we directly compare the abilities of the Promega CellTiter-Glo® Luminescent Cell Viability Assay, an Ultra-Glo™ rLuciferase-based luminescent ATP detection system, and the Perkin Elmer ATPlite™ 1step assay to resist common commercial luciferase inhibitors. The CellTiter-Glo® Assay has greater than 80% activity for 5 out of the 7 compounds at an inhibitor concentration of 10μM; whereas, ATPlite™ 1step has 80% activity for only 1 out of 7 compounds at the same inhibitor concentrations.
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