Characterization of an IgG-Cleaving Protease from Streptococcus equi with Improved Activity Against Mouse IgGs
Part # PS260
Chris Hosfield1, Philip Compton2, Luca Fornelli2, Paul Thomas2, Neil L. Kelleher2, Michael Rosenblatt1 and Marjeta Urh1
1Promega Corporation, 2800 Woods Hollow Rd Madison, WI 53711; 2Proteomics Center of Excellence, Northwestern University, Evanston, IL
IdeZ Protease was originally identified in S. equi subsp, zooepidemicus. Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.
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