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CellTiter-Glo® 2.0: A Novel Luminescent Cell Viability Assay with Greatly Enhanced Storage Stability

Part # PS196


Michael P. Valley1, James Unch2 , Poncho L. Meisenheimer2, James J. Cali1, and Dan F. Lazar1
1Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711-5399
2Promega Biosciences, 277 Granada Dr., San Luis Obispo, CA 93401

Here we report on the attributes of a novel ATP detection reagent for cell viability with all of the assay performance of the previous CellTiter-Glo® Reagent, but now with markedly enhanced stability as a single component in a liquid format. These new features provide for much greater ease-of-use in that storage of the reagent at 4°C eliminates the requirement for reagent thawing and minimizes temperature equilibration time. The enhanced stability also means that reagent performance is maintained at room temperature during extended assay operations, and any unused reagent can be retained for future use by simply returning it to 4°C storage rather than having to freeze or discard it.

Printed in USA 11/13.