Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

A Rapid, Automated Immunoaffinity LC-MS/MS Workflow

Part # PS320


Michael Rosenblatt1, Gregory Barrett-Wilt2, Nidhi Nath1, Lyndsey Jager1, Marjeta Urh1
1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711 ; 2University of Wisconsin, 425 Henry Mall Madison WI 53706

The analysis of Biotherapeutic antibody metabolism in various animal models is a critical requirement during drug development. As the number of potential antibody targets have increased targets as well as Biosimilar development, there is an immediate need to increase sample throughput. Toward this end, we have developed a streamlined workflow that involves mAb enrichment, using highcapacity magnetic streptavidin particles, followed by proteolysis using a commercially available Rapid Digestion kit. The rapid enrichment/digestion step combined with direct LC-MS/MS analysis (no offline cleanup required) allows for protocols that can be carried out in very short time periods (as short as 2–3 hours). The protocol is very simple, requires routine laboratory equipment, and can be easily automated.

Printed in USA.